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. Author manuscript; available in PMC: 2024 Jun 20.
Published in final edited form as: Nature. 2023 Dec 6;625(7993):166–174. doi: 10.1038/s41586-023-06797-9

Figure 2. Local IL-4 signaling in bone marrow fuels immunosuppressive myelopoiesis.

Figure 2.

(a) Relative expression of statistically significant maturation-associated genes in lung monocyte populations from Il4ra+/+ and Il4raΔMs4a3 tumor-bearing mice (n=3 mice per group). (b) Number of bulk GMPs per femur in naïve and tumor-bearing Il4ra+/+ and Il4raΔMs4a3 mice (n=8, 8, 23, 23 mice per group) Pooled from three independent experiments. (c) pSTAT6 staining in indicated BM myeloid progenitors in naïve (n=5) and KP tumor-bearing (n=6) mice. Representative of two independent experiments. (d) Proportion of WT (CD45.1) and Il4raΔMs4a3 (CD45.2) cells in indicated cell populations in mice reconstituted with 1:1 (WT : Il4raΔMs4a3 ) BM cells 12 weeks post-transplant (n=5 mice per group). One experiment. (e) Number of indicated hematopoietic progenitor populations in BM of vehicle and IL-4c injected mice (n=10 mice per group). Pooled from two independent experiments. (f) Number of monocytes per ml of blood in vehicle and IL-4c injected mice (n=5 mice per group). Representative of two independent experiments. (g) Curated gene lists showing indicate genes in GMP, pre-monocyte, and monocyte clusters of IL-4c and KP-treated mice relative to naïve controls. Unpaired two-tailed Student’s t-test. Data are mean ± s.d. HSC-LT, Long term hematopoietic stem cell. HSC-ST, Short term hematopoietic stem cell.