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. 2024 Jan 19;29(5):1338–1349. doi: 10.1038/s41380-024-02413-y

Fig. 5. Normalizing MG-BDNF during the juvenile period does not impair sociability or abnormal inhibitory inputs in the mPFC.

Fig. 5

a–l The Iba1-tTA(+)::Bdnf(tetO/+) mice were administered doxycycline from p21 at weaning to normalize MG-BDNF. Bdnf(tetO/+) mice were also administered doxycycline from p21 as the control. Behavioral and electrophysiological experiments were started at p61. b Normalizing MG-BDNF from p21 did not reduce sociability in adult Iba1-tTA(+)::Bdnf(tetO/+) mice in the three-chamber social test. Both groups had no differences in the social interaction score (U = 57, p = 0.2701, Mann–Whitney U test, Bdnf(tetO/+): n = 12, Iba1-tTA(+)::Bdnf(tetO/+): n = 13) (left) or social investigation time (F1,23(interaction) = 2.626, p = 0.1187, two-way ANOVA, Bdnf(tetO/+): n = 12, Iba1-tTA(+)::Bdnf(tetO/+): n = 13) (right). S, social; O, object. c Representative traces recorded from mPFC pyramidal cells at 200-pA injection. d No differences existed between Bdnf(tetO/+) and Iba1-tTA(+)::Bdnf(tetO/+) mice treated with doxycycline from p21 in the spike frequency (U = 110, p = 0.5192, Mann–Whitney U test) (left), spike amplitude (U = 120, p = 0.7944, Mann–Whitney U test) (middle), or threshold (t(30) = 2.026, p = 0.0517, unpaired two-tailed Student’s t test) (right) at 200-pA injection (n  =  17 cells from three biologically independent Bdnf(tetO/+) mice, n  =  15 cells from four biologically independent Iba1-tTA(+)::Bdnf(tetO/+) mice). e Representative traces of spontaneous EPSCs. f No differences were observed between Bdnf(tetO/+) and Iba1-tTA(+)::Bdnf(tetO/+) mice treated with doxycycline from p21 in sEPSC frequency (U = 85, p = 0.2671, Mann–Whitney U test) (left) or amplitude (U = 89, p = 0.3453, Mann–Whitney U test) (right). g Representative traces of spontaneous IPSCs. (h) Normalizing MG-BDNF from p21 did not increase sIPSC frequency (U = 107, p = 0.8381, Mann–Whitney U test) (left) or amplitude (U = 69, p = 0.0742, Mann–Whitney U test) (right) in Iba1-tTA(+)::Bdnf(tetO/+) mice. f, h n  =  15 cells from five biologically independent Bdnf(tetO/+) mice, n  =  15 cells from three biologically independent Iba1-tTA(+)::Bdnf(tetO/+) mice. i Representative traces of miniature EPSCs. j No differences existed between Bdnf(tetO/+) and Iba1-tTA(+)::Bdnf(tetO/+) mice treated with doxycycline from p21 in mEPSC frequency (t(28) = 1.988, p = 0.0566, unpaired two-tailed Student’s t test) (left) or amplitude (t(28) = 1.729, p = 0.0948, unpaired two-tailed Student’s t test) (right). k Representative traces of miniature IPSCs. l Normalizing MG-BDNF from p21 did not increase mIPSC frequency (t(28) = 0.01783, p = 0.9859, unpaired two-tailed Student’s t test) (left) or amplitude (U = 76, p = 0.1370, Mann–Whitney U test) (right) in Iba1-tTA(+)::Bdnf(tetO/+) mice. j, l n  =  15 cells from five biologically independent Bdnf(tetO/+) mice, n  =  15 cells from three biologically independent Iba1-tTA(+)::Bdnf(tetO/+) mice. Data are presented as the mean ± SEM. 2 M: two months of age, control: Bdnf(tetO/+) mice, Iba1-Bdnf: Iba1-tTA(+)::Bdnf(tetO/+) mice.