Figure 1.

T lymphocytes from SPMS patients induce excitotoxic effects in a heterologous chimeric ex vivo model. (A) Schematic representation of heterologous T-cell chimeric MS model. Voltage-clamp recordings from MSNs of murine corticostriatal slices incubated with HC-T cells or SPMS-T cells. Representative peaks of electrophysiological recordings in the two experimental conditions are shown in the top panel. The graphs show the kinetic properties of sEPSCs in the two experimental conditions. The rise time, decay time, and half-width were altered in SPMS patients compared to HS (HC: N =6, n =55 cells, SPMS: N =7, n =40 cells). Each dot in the graphs represents the value of a single cell time rise, decay time, and half-width. HC and SPMS populations are labeled by white and grey color dots, respectively. (B) The differences observed in graphs A were maintained even considering the mean values of the parameters for each subject. Dotted lines refer to control conditions. Data are expressed as mean ± SEM. Statistical analysis was performed by Unpaired T-test; * p < 0.05; ** p < 0.01; *** p < 0.001. N represents numbers of patients and n represents numbers of cells.