TABLE 1.

Main chemical constituents and relative detection methods of Danshen-Shanzha Formula (DSF).

No.	Constituents	Extraction solvent	Detection method	Source	References
Tanshinones
1	Tanshinone I	70% methanol	HPLC-PAD/HPLC-MS	Danshen	Liang et al. (2017)
2	Dihydrotanshinone	50% ethanol	HPLC-MS/MS	Danshen	Pang et al. (2018)
3	Tanshinone IIA	supercritical CO2 fluid	HPLC-MS	Danshen	Yuan et al. (2020)
		70% methanol	HPLC-PDA/HPLC-MS	Danshen	Liang et al. (2017)
		50% ethanol	HPLC-MS/MS	Danshen	Pang et al. (2018)
4	Tanshinone IIB	70% methanol	HPLC-PDA/HPLC-MS	Danshen	Liang et al. (2017)
		50% ethanol	HPLC-MS/MS	Danshen	Pang et al. (2018)
5	Cryptotanshinone	supercritical CO2 fluid	HPLC-MS	Danshen	Yuan et al. (2020)
		70% methanol	HPLC-PDA/HPLC-MS	Danshen	Liang et al. (2017)
		50% ethanol	HPLC-MS/MS	Danshen	Pang et al. (2018)
6	Neocryptotanshinone	50% ethanol	HPLC-MS/MS	Danshen	Pang et al. (2018)
7	Methyl tanshinonate	70% methanol	HPLC-PDA/HPLC-MS	Danshen	Liang et al. (2017)
		50% ethanol	HPLC-MS/MS	Danshen	Pang et al. (2018)
8	Tanshindiol B	70% methanol	HPLC-PDA/HPLC-MS	Danshen	Liang et al. (2017)
9	Tanshindiol C	70% methanol	HPLC-PDA/HPLC-MS	Danshen	Liang et al. (2017)
10	3a-Hydroxymethylenetanshinquinone	70% methanol	HPLC-PDA/HPLC-MS	Danshen	Liang et al. (2017)
11	Tanshinol B	50% ethanol	HPLC-MS/MS	Danshen	Pang et al. (2018)
		70% methanol	HPLC-PDA/HPLC-MS	Danshen	Liang et al. (2017)
12	Tanshinaldehyde	70% methanol	HPLC-PDA/HPLC-MS	Danshen	Liang et al. (2017)
13	Trijuganone B	70% methanol	HPLC-PDA/HPLC-MS	Danshen	Liang et al. (2017)
14	Danshenxinkun A	70% methanol	HPLC-PDA/HPLC-MS	Danshen	Liang et al. (2017)
15	Miltirone	70% methanol	HPLC-PDA/HPLC-MS	Danshen	Liang et al. (2017)
		50% ethanol	HPLC-MS/MS	Danshen	Pang et al. (2018)
16	Neoprzewaquinone A	50% ethanol	HPLC-MS/MS	Danshen	Pang et al. (2018)
17	Danshenxinkun B	50% ethanol	HPLC-MS/MS	Danshen	Pang et al. (2018)
18	Hydroxytanshinone IIA	50% ethanol	HPLC-MS/MS	Danshen	Pang et al. (2018)
19	Methyl dihydronortanshinonate	70% methanol	HPLC-PDA/HPLC-MS	Danshen	Liang et al. (2017)
		50% ethanol	HPLC-MS/MS	Danshen	Pang et al. (2018)
20	Przewaquinone B	50% ethanol	HPLC-MS/MS	Danshen	Pang et al. (2018)
21	Hydroxycryptotanshinone	50% ethanol	HPLC-MS/MS	Danshen	Pang et al. (2018)
22	Salvianonol	50% ethanol	HPLC-MS/MS	Danshen	Pang et al. (2018)
23	Dehydromiltirone	50% ethanol	HPLC-MS/MS	Danshen	Pang et al. (2018)
24	Dehydrotanshinone IIA	50% ethanol	HPLC-MS/MS	Danshen	Pang et al. (2018)
Phenylpropanoids
25	Danshensu	50% ethanol	HPLC-MS/MS	Danshen	Pang et al. (2018)
		supercritical CO2 fluid	HPLC-MS	Danshen	Yuan et al. (2020)
		70% methanol	HPLC-PDA/HPLC-MS	Danshen	Liang et al. (2017)
26	Protocatechuic acid	supercritical CO2 fluid	HPLC	Danshen	Yuan et al. (2020)
		70% methanol	HPLC-PDA/HPLC-MS	Danshen	Liang et al. (2017)
		50% ethanol	HPLC-MS/MS	Danshen	Pang et al. (2018)
		80% methanol	HPLC-PDA	Shanzha	Sun et al. (2021)
27	Protocatechuic aldehyde	supercritical CO2 fluid	HPLC-MS	Danshen	Yuan et al. (2020)
		70% methanol	HPLC-PDA/HPLC-MS	Danshen	Liang et al. (2017)
		50% ethanol	HPLC-MS/MS	Danshen	Pang et al. (2018)
28	Caffeic acid	70% methanol	HPLC-PDA/HPLC-MS	Danshen	Liang et al. (2017)
		50% ethanol	HPLC-MS/MS	Danshen	Pang et al. (2018)
		80% methanol	HPLC-PDA	Shanzha	Sun et al. (2021)
29	Rosmarinic acid	50% ethanol	HPLC-MS/MS	Danshen	Pang et al. (2018)
30	Salvianolic acid A	70% methanol	HPLC-PDA/HPLC-MS	Danshen	Liang et al. (2017)
		water	HPLC	Danshen	Chen et al. (2013)
		50% ethanol	HPLC-MS/MS	Danshen	Pang et al. (2018)
31	Salvianolic acid B	supercritical CO2 fluid	HPLC-MS	Danshen	Yuan et al. (2020)
		70% methanol	HPLC-PDA/HPLC-MS	Danshen	Liang et al. (2017)
		water	HPLC-PDA	Danshen	Chen et al. (2013)
		50% ethanol	HPLC-MS/MS	Danshen	Pang et al. (2018)
32	Salvianolic acid C	50% ethanol	HPLC-MS/MS	Danshen	Pang et al. (2018)
33	Salvianolic acid D	water	HPLC/Q-TOF-MS	Danshen	Wu et al. (2018)
34	Salvianolic acid E	70% methanol	HPLC-PDA/HPLC-MS	Danshen	Liang et al. (2017)
		50% ethanol	HPLC-MS/MS	Danshen	Pang et al. (2018)
35	Salvianolic acid F	70% methanol	HPLC-PDA/HPLC-MS	Danshen	Liang et al. (2017)
		50% ethanol	HPLC-MS/MS	Danshen	Pang et al. (2018)
36	Salvianolic acid G	70% methanol	HPLC-PDA/HPLC-MS	Danshen	Liang et al. (2017)
37	Salvianolic acid H	50% ethanol	HPLC-MS/MS	Danshen	Pang et al. (2018)
38	Salvianolic acid I	50% ethanol	HPLC-MS/MS	Danshen	Pang et al. (2018)
39	Salvianolic acid L	70% methanol	HPLC-PDA/HPLC-MS	Danshen	Liang et al. (2017)
40	ProcyanidinB2	water	HPLC-PDA	Danshen	Chen et al. (2013)
41	Isosalvianolic acid C	50% ethanol	HPLC-MS/MS	Danshen	Pang et al. (2018)
42	Chlorogenic acid	water	HPLC-PDA	Danshen	Chen et al. (2013)
		75% ethanol	UPLC/ESI-TOF-MS	Shanzha	Qiao et al. (2014)
43	Ferulic acid	50% ethanol	HPLC-MS/MS	Danshen	Pang et al. (2018)
		80% methanol	HPLC-PDA	Shanzha	Sun et al. (2021)
44	Isoferulic acid	50% ethanol	HPLC-MS/MS	Danshen	Pang et al. (2018)
45	Lithospermic acid	water	HPLC-PDA	Danshen	Chen et al. (2013)
		70% methanol	HPLC-PDA/HPLC-MS	Danshen	Liang et al. (2017)
46	Dimethyl lithospermate	50% ethanol	HPLC-MS/MS	Danshen	Pang et al. (2018)
47	Dihydrocaffeic acid	80% methanol	HPLC-PDA	Shanzha	Sun et al. (2021a)
48	Cinnamic acid	80% methanol	HPLC-PDA	Shanzha	Sun et al. (2021b)
Triterpenoids
49	Maslinic acid	ethyl acetate	NMR and MS	Danshen	Tung et al. (2017)
		methanol	HPLC-CAD	Shanzha	Du et al. (2023)
50	Ursolic acid	ethyl acetate	NMR and MS	Danshen	Tung et al. (2017)
		methanol	HPLC-CAD	Shanzha	Du et al. (2023)
51	Oleanolic acid	ethyl acetate	NMR and MS	Danshen	Tung et al. (2017)
		methanol	HPLC-CAD	Shanzha	Du et al. (2023)
52	Corosolic acid	methanol	HPLC-CAD	Shanzha	Du et al. (2023)
53	Betulinic acid	methanol	HPLC-CAD	Shanzha	Du et al. (2023)
54	Pomolic acid	95% ethanol	NMR	Shanzha	Zhu et al. (2022)
55	Tormentic acid	95% ethanol	NMR	Shanzha	Zhu et al. (2022)
56	Euscaphic acid	95% ethanol	NMR	Shanzha	Zhu et al. (2022)
57	Fupenzic acid	95% ethanol	NMR	Shanzha	Zhu et al. (2022)
58	α-Amyrin	95% ethanol	NMR	Shanzha	Zhu et al. (2022)
59	β-Amyrin	95% ethanol	NMR	Shanzha	Zhu et al. (2022)
60	3-Epifriedelinol	95% ethanol	NMR	Shanzha	Zhu et al. (2022)
Flavonoids
61	Hyperoside	water	HPLC-PDA	Shanzha	Chen et al. (2013)
		50% ethanol	UPLC/Q-TOF-MS	Shanzha	Ao et al. (2020)
62	Quercetin	methanol	UPLC-Q-TOF-MS/MS	Shanzha	Ning et al. (2021)
		50% ethanol	UPLC/Q-TOF-MS	Shanzha	Ao et al. (2020)
63	Isoquercetin	water	HPLC-PDA	Shanzha	Chen et al. (2013)
		50% ethanol	UPLC/Q-TOF-MS	Shanzha	Ao et al. (2020)
64	Vitexin	methanol	UPLC-Q-TOF-MS/MS	Shanzha	Ning et al. (2021)
65	Catechin	50% ethanol	UPLC/Q-TOF-MS	Shanzha	Ao et al. (2020)
66	Epicatechin	methanol	UPLC-Q-TOF-MS/MS	Shanzha	Ning et al. (2021)
		50% ethanol	UPLC/Q-TOF-MS	Shanzha	Ao et al. (2020)
67	Hesperidin	50% ethanol	UPLC/Q-TOF-MS	Shanzha	Ao et al. (2020)
68	Rutin	methanol	UPLC-Q-TOF-MS/MS	Shanzha	Ning et al. (2021)
		water	HPLC	Shanzha	Chen et al. (2013)
		50% ethanol	UPLC/Q-TOF-MS	Shanzha	Ao et al. (2020)
69	Luteolin	50% ethanol	UPLC/Q-TOF-MS	Shanzha	Ao et al. (2020)
70	Vitexin rhamnoside	50% ethanol	UPLC/Q-TOF-MS	Shanzha	Ao et al. (2020)
71	Vitexin-2′-O-rhamnoside	methanol	UPLC-Q-TOF-MS/MS	Shanzha	Ning et al. (2021)
72	Vitexin-4′-O-glucoside	methanol	UPLC-Q-TOF-MS/MS	Shanzha	Ning et al. (2021)
73	Proanthocyanidin B2	50% ethanol	UPLC/Q-TOF-MS	Shanzha	Ao et al. (2020)
		75% ethanol	UPLC/ESI-TOF-MS	Shanzha	Qiao et al. (2014)
74	Proanthocyanidin B3	75% ethanol	UPLC/ESI-TOF-MS	Shanzha	Qiao et al. (2014)
Organic acids
75	Citric acid	methanol	UPLC-Q-TOF-MS/MS	Shanzha	Ning et al. (2021)
		water	HPLC-PDA	Shanzha	Jiang, (2021)
76	Malic acid	water	HPLC-PDA	Shanzha	Jiang, (2021)
77	Tartaric acid	water	HPLC-PDA	Shanzha	Jiang, (2021)
78	Gallic acid	water	HPLC-PDA	Shanzha	Jiang, (2021)
79	Succinic acid	water	HPLC-PDA	Shanzha	Jiang, (2021)
80	Oxalic acid	water	HPLC-PDA	Shanzha	Jiang, (2021)
81	Linoleic acid	methanol	UPLC-Q-TOF-MS/MS	Shanzha	Ning et al. (2021)
82	Coumalic acid	methanol	UPLC-Q-TOF-MS/MS	Shanzha	Ning et al. (2021)
83	Phlogistic acid	80% methanol	HPLC-PDA	Shanzha	Sun et al. (2021a)
Volatile oil
84	Vanillin	methanol	UPLC-Q-TOF-MS/MS	Shanzha	Ning et al. (2021)