Fig. 8. In vivo therapeutic efficacies of Imi@LUN@RBPP545 and Amp@UPSN@CBDSA97 against resistant pathogens.
a Survival rates of mice in the CRKP-induced mouse pneumonia model (n = 10 biological replicates). Survival was analyzed by the Log-rank (Mantel-Cox) test. ns, no significance; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. b–f Treated with Imi@LUN@RBPP545 (20 mg/kg) significantly reduced the bacterial load of organs of the CRKP-induced pneumonia mouse relative to equivalent doses of untargeted imipenem nanoparticles or of free imipenem. At 24 h post-infection, the mice (n = 6) were euthanized by cervical dislocation. Bacterial loads (Log10 c.f.u. per gram of K. pneumoniae) of the lung (b), heart (c), liver (d), spleen (e), and kidney (f) were counted. Data are presented as mean ± standard deviation (n = 6 biological replicates). The statistical significance of the data was assessed using one-way ANOVA followed by Tukey’s multiple comparisons test. ns, no significance; **p < 0.01; ***p < 0.001; ****p < 0.0001. g Survival rates of mice in the MRSA-induced mouse pneumonia model (n = 10 biological replicates). Survival was analyzed by the Log-rank (Mantel-Cox) test. ns, no significance; **p < 0.01; ***p < 0.001; ****p < 0.0001. h–l Treated with Amp@UPSN@CBDSA97 (20 mg/kg) significantly reduced the bacterial load of organs of the MRSA-induced pneumonia mouse relative to equivalent doses of untargeted ampicillin nanoparticles or of free ampicillin. At 24 h post-infection, the mice (n = 6) were euthanized by cervical dislocation. Bacterial loads (Log10 c.f.u. per gram of S. aureus) of the lung (h), heart (i), liver (j), spleen (k), and kidney (l) were counted. Data are presented as mean ± standard deviation (n = 6 biological replicates). The statistical significance of the data was assessed using one-way ANOVA followed by Tukey’s multiple comparisons test. ns, no significance; ***p < 0.001; ****p < 0.0001. Source data are provided as a Source Data file.