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. 2024 Jun 7;137(20):jcs261828. doi: 10.1242/jcs.261828

Fig. 4.

Fig. 4.

The frequency of VAMP3 exocytosis in primary rat oligodendrocytes is much lower than that of human melanoma cells. (A) Representative image of segmented oligodendrocyte (OL) and event detail. Scale bar: 10 µm. (B) Quantification of exocytosis with manual (0.0038±0.0005) and automated (0.0041±0.0006) detection (mean±s.e.m., n=21 cells). Frequencies reported as number of events/µm2/min. Cells expressing both VAMP3–sepHluorin and tetanus toxin light chain (TeNT) were quantified manually (0.00012±3.6×10−5) and automated (0.0002±5.6×10−5). (C) Representative image of 1205Lu human melanoma cell line and example exocytic trace. Scale bar: 10 µm. (D) Frequency of VAMP3–sepHluorin exocytosis in primary rat OL (0.0041±0.0006) and 1205Lu cells (0.12±0.011, mean±s.e.m., n=21). (E) Frequency of exocytosis in 1205Lu cells imaged at 2 Hz (0.067±0.0077, mean±s.e.m., n=23) and 4 Hz (0.12±0.011, mean±s.e.m., n=21). For box plots, the box represents the 25–75th percentiles, and the median is indicated. The whiskers show the smallest and largest non-outlier values (within 1.5×IQR from the Q1 and Q3 boundaries).