Fig. 5.

Persistence of exocytic events in developing and mature neurons. (A) Left, representative image of primary rat hippocampal neurons (DIV 12–14). The inset shows a detail of the network. Right, an example image series centered on an event. The mean intensity in each image and the fit to the intensity values (green line) from the start of the event are plotted above. Circles indicate that the frame meets the criterium for a Gaussian function fit. A red circle marks the beginning of the event. Squares do not meet the criterium for a Gaussian function. (B) Histograms of the time of the event plateau between mature (n=223) and immature neurons (n=203). Scale bars: 10 μm.