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. 2024 May 3;300(6):107338. doi: 10.1016/j.jbc.2024.107338

Figure 2.

Figure 2

Identification of MAIT cells in multiple species using species-specific MR1–5-OP-RU and MR1–6-FP tetramers.A, representative flow cytometry plots from staining of human PBMC, cattle PBMC, pig-tailed macaque PBMC, mouse lung-, spleen- and blood-derived lymphocytes, and pig PBMC with MR1–5-OP-RU and MR1–6-FP tetramers of the corresponding species. B, frequencies of species-matched MR1–5-OP-RU and MR1–6-FP tetramer+ MAIT cells from human CD3+ PBMC (n = 4), cattle CD3+ PBMC (n = 4), pig-tailed macaque CD8+ PBMC (n = 5), mouse lungs, spleen, and whole blood-derived αβTCR+ lymphocytes (n = 6), and pig CD3+ PBMC (n = 3). Mean ± SEM is depicted. Experiments were performed once in pig-tailed macaque and mouse and twice in all other species. 5-OP-RU, 5-(2-oxopropylideneamino)-6-d-ribitylaminouracil; 6-FP, 6-formylpterin; MAIT, mucosal-associated invariant T; MR1, major histocompatibility complex-I related protein 1; PBMC, peripheral blood mononuclear cell; TCR, T cell receptor.