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. 2024 May 3;300(6):107338. doi: 10.1016/j.jbc.2024.107338

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© 2024 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Frequency and TCRα usage of putative MAIT cells in cattle, sheep and pig PBMC.A, frequencies of TRAV1⁺ (I) and putative MAIT TCR CDR3α sequence⁺ (II) of total TRA bulk sequenced cattle, sheep, and pig PBMC (n = 2). B, frequency of TRAJ33, TRAJ12, or TRAJ20 gene segment usage among TRAV1⁺TRA sequences from cattle, sheep, and pig PBMC. C, relative frequency and sequence of putative MAIT TCR CDR3α-loops in each animal. D, sequence logo (Seq2Logo) of the amino acid weighting of the CDR3α-loops from putative MAIT TCRs in each species (note that the Y95, highlighted in green, is conserved between all species). MAIT, mucosal-associated invariant T; PBMC, peripheral blood mononuclear cell; TCR, T cell receptor.