Table 2.
MR1–6-FP tetramer staining characteristics
| Cellular population | Parameter | Frequency MR1–6-FP tetramer+ |
Fold change gMFI (MR1–6-FP tetramer+/MR1–6-FP tetramer−) |
||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Hum | Cattle | Mac | Mouse | Pig | Hum | Cattle | Mac | Mouse | Pig | ||
| Human PBMC CD3+ | Value # | 8 | 8 | 8 | 8 | 8 | 8 | 8 | 8 | 8 | 8 |
| Min | 0.01 | 0.25 | 0.04 | 0.03 | 0.01 | 8.32 | 8.48 | 6.96 | 13.81 | 6.70 | |
| Max | 0.19 | 2.86 | 0.28 | 0.21 | 0.06 | 45.14 | 28.22 | 20.03 | 64.83 | 89.15 | |
| Range | 0.18 | 2.61 | 0.24 | 0.18 | 0.06 | 36.82 | 19.74 | 13.08 | 51.02 | 82.46 | |
| Mean | 0.05 | 1.02 | 0.09 | 0.09 | 0.03 | 26.03 | 19.45 | 12.79 | 27.76 | 31.97 | |
| SD | 0.06 | 0.87 | 0.08 | 0.07 | 0.02 | 13.78 | 5.91 | 3.76 | 17.38 | 31.47 | |
| SEM | 0.02 | 0.31 | 0.03 | 0.02 | 0.01 | 4.87 | 2.09 | 1.33 | 6.15 | 11.13 | |
| Cattle PBMC CD3+ MHC-II- | Value # | 8 | 8 | 8 | 8 | 8 | 8 | 8 | 8 | 8 | 8 |
| Min | 0.04 | 0.07 | 0.01 | 0.04 | 0.02 | 23.60 | 17.25 | 24.35 | 35.19 | 26.13 | |
| Max | 0.10 | 0.31 | 0.07 | 0.10 | 0.11 | 145.30 | 86.66 | 177.60 | 110.60 | 81.45 | |
| Range | 0.06 | 0.23 | 0.06 | 0.06 | 0.09 | 121.70 | 69.41 | 153.20 | 75.44 | 55.32 | |
| Mean | 0.06 | 0.19 | 0.04 | 0.07 | 0.06 | 68.19 | 45.96 | 95.10 | 60.84 | 51.61 | |
| SD | 0.02 | 0.09 | 0.03 | 0.03 | 0.03 | 45.14 | 26.21 | 61.15 | 26.04 | 24.05 | |
| SEM | 0.01 | 0.03 | 0.01 | 0.01 | 0.01 | 15.96 | 9.27 | 21.62 | 9.21 | 8.50 | |
| Pig-tailed macaque PBMC CD8+ | Value # | 5 | 3 | 4 | 5 | 5 | 5 | 3 | 4 | 5 | 5 |
| Min | 0.11 | 0.25 | 0.11 | 1.26 | 0.13 | 12.74 | 23.14 | 32.86 | 17.59 | 14.16 | |
| Max | 0.24 | 0.35 | 0.75 | 2.01 | 0.19 | 20.43 | 27.19 | 121.10 | 20.44 | 16.33 | |
| Range | 0.14 | 0.10 | 0.64 | 0.75 | 0.06 | 7.69 | 4.05 | 88.28 | 2.85 | 2.17 | |
| Mean | 0.18 | 0.31 | 0.33 | 1.64 | 0.16 | 15.14 | 25.76 | 68.56 | 18.79 | 15.10 | |
| SD | 0.06 | 0.05 | 0.29 | 0.27 | 0.02 | 3.06 | 2.27 | 40.63 | 1.46 | 0.90 | |
| SEM | 0.03 | 0.03 | 0.15 | 0.12 | 0.01 | 1.37 | 1.31 | 20.31 | 0.65 | 0.40 | |
| Boosted mouse spleen TCRβ+ | Value # | 7 | 7 | 7 | 6 | 7 | 6 | 5 | 5 | 5 | 5 |
| Min | 0.10 | 0.12 | 0.08 | 0.12 | 0.18 | 9.16 | 7.883 | 8.26 | 8.62 | 8.30 | |
| Max | 0.38 | 0.57 | 0.21 | 0.40 | 1.33 | 19.64 | 13.57 | 15.33 | 47.16 | 10.88 | |
| Range | 0.28 | 0.45 | 0.13 | 0.28 | 1.14 | 10.48 | 5.691 | 7.07 | 38.54 | 2.58 | |
| Mean | 0.23 | 0.22 | 0.13 | 0.26 | 0.49 | 12 | 10.66 | 11.28 | 18.40 | 9.18 | |
| SD | 0.10 | 0.16 | 0.05 | 0.11 | 0.41 | 3.91 | 2.216 | 2.87 | 16.29 | 1.08 | |
| SEM | 0.04 | 0.06 | 0.02 | 0.04 | 0.15 | 1.596 | 0.991 | 1.28 | 7.29 | 0.48 | |
| Sheep PBMC CD8+ | Value # | 6 | 6 | 6 | 6 | 6 | 6 | 6 | 6 | 6 | 5 |
| Min | 0.27 | 0.14 | 0.11 | 0.17 | 0.08 | 50.91 | 38.64 | 22.05 | 95.74 | 23.10 | |
| Max | 1.93 | 1.63 | 0.96 | 1.26 | 0.90 | 100.20 | 101.30 | 175.40 | 403.20 | 85.35 | |
| Range | 1.66 | 1.48 | 0.86 | 1.09 | 0.82 | 49.28 | 62.67 | 153.40 | 307.50 | 62.25 | |
| Mean | 0.81 | 0.74 | 0.53 | 0.51 | 0.44 | 65.66 | 61.28 | 92.80 | 245.90 | 46.42 | |
| SD | 0.58 | 0.51 | 0.35 | 0.40 | 0.36 | 19.87 | 23.12 | 63.28 | 106.70 | 25.04 | |
| SEM | 0.24 | 0.21 | 0.14 | 0.16 | 0.15 | 8.11 | 9.44 | 25.83 | 43.57 | 11.20 | |
The bold values highlight data from species matched staining, e.g., human samples stained with human tetramer, whilst regular font shows data from species mismatched font, e.g., human samples stained with cattle tetramer. In the matching figures, bar graphs of species-matched staining are in red, while bar graphs of species mismatched staining are in black.