Table 3.
Putative MAIT TCR sequences
| Animal | TRAV usage | TRAJ usage | CDR3α sequence | Number of transcripts | % of total putative MAIT cell sequences | % of total TCR CDR3α sequences | Combined % total TCR CDR3α sequences |
|---|---|---|---|---|---|---|---|
| Cow 1 | TRAV1 | TRAJ33 | CVVMDGNYQWIW | 159 | 56.8 | 0.32 | 0.56 |
| TRAV1 | TRAJ33 | CVVIDGNYQWIW | 121 | 43.2 | 0.24 | ||
| Cow 2 | TRAV1 | TRAJ33 | CVVMDGNYQWIW | 181 | 79 | 0.31 | 0.39 |
| TRAV1 | TRAJ33 | CVVIDGNYQWIW | 42 | 18.3 | 0.072 | ||
| TRAV1 | TRAJ33 | CVVTDGNYQWIW | 2 | 0.9 | 0.0034 | ||
| TRAV1 | TRAJ33 | CVVADGNYQWIW | 1 | 0.4 | 0.0017 | ||
| TRAV1 | TRAJ33 | CAAIAGNYQWIW | 3 | 1.3 | 0.0051 | ||
| Sheep 1 | TRAV1 | TRAJ33 | CAVMDGNYRLIW | 36 | 39.6 | 0.14 | 0.35 |
| TRAV1 | TRAJ33 | CAVIDGNYRLIW | 34 | 37.4 | 0.13 | ||
| TRAV1 | TRAJ33 | CVVKDGNYQWIW | 17 | 18.7 | 0.066 | ||
| TRAV1 | TRAJ33 | CVVQDGNYQWIW | 1 | 1.1 | 0.0039 | ||
| TRAV1 | TRAJ33 | CVVMNGNYQWIW | 1 | 1.1 | 0.0039 | ||
| TRAV1 | TRAJ33 | CVVRDGNYQWIW | 1 | 1.1 | 0.0039 | ||
| TRAV1 | TRAJ20 | CVVVLNNYKLTF | 1 | 1.1 | 0.0039 | ||
| Sheep 2 | TRAV1 | TRAJ33 | CAVMDGNYRLIW | 27 | 87.1 | 0.097 | 0.11 |
| TRAV1 | TRAJ33 | CAVLDGNYQLIW | 4 | 12.9 | 0.014 | ||
| Pig 1 | TRAV1 | TRAJ33 | CAVKDSSYQLIW | 1 | 50 | 0.0027 | 0.0055 |
| TRAV1 | TRAJ33 | CAVSDGGYQLIW | 1 | 50 | 0.0027 | ||
| Pig 2 | TRAV1 | TRAJ33 | CAVLDSSYQLIW | 1 | 100 | 0.0024 | 0.0024 |