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(A) Schematic diagram of the preparation of VCRS liposomes. The molecular structure of (B) PC and (C) ESM. The effect of (D) drug-to-lipid ratio and (E) concentration of citrate buffer on the encapsulation efficiency of VCRS liposomes (n = 3). (F) The effect of freeze-thaw cycle on the particle size and encapsulation efficiency of VCRS liposomes (n = 3). The effect of drug loading (D) temperature and (E) incubation time on the encapsulation efficiency of VCRS liposomes (n = 3).
