TABLE 1.

Amino acid sequence of TCR β-chain junction of T-cell clones derived from three HCMV⁺ donors and recognizing either pp65 or IE1^a

Donor	HLA restriction	Amino acid position in:		TCR β-chain sequence					No. of clones
		IE1	pp65	Vβ		CDR3	Jβ
A	A1		353–375	Vβ1S1	CAS	SPRGFDAGGYWLD	LRF	Jβ1.2	1
Total									1
B	A2		485–507	Vβ6S4A1	CAS	SYGQNPLNTE	AFF	Jβ1.1	1
B	A2		485–507	Vβ14S1	CAS	SLEGYTE	AFF	Jβ1.1	1
Total									2
C	A2	309–331		Vβ1S1	CAS	SVDGTGGALGNT	IYF	Jβ1.3	1
C	A2	309–331		Vβ4S1	CSV	EXSPPGEEWWLT	LRF	Jβ1.6	1
C	A2	309–331		Vβ210S1	CAW	SWGDSTNSP	LHF	Jβ1.6	19
C	A2	309–331		Vβ21S3	CAS	SLVGGTGSVSY	QFF	Jβ2.1	1
Total									22
C	B18	188–210, 199–221		Vβ3S1	CAS	SLLMATNEK	LFF	Jβ1.4	1
C	B18	188–210, 199–221		Vβ6S5	CAS	SYRANYE	QYF	Jβ2.7	2
C	B18	188–210, 199–221		Vβ9S2	CAS	SQAVASGGGRSE	QFF	Jβ2.1	1
C	B18	188–210, 199–221		Vβ22S1	CAS	VTGSTDT	QYF	Jβ2.3	7
C	B18	188–210, 199–221		Vβ22S1	CAS	SDGTAYE	QYF	Jβ2.7	10
C	B18	265–287		Vβ7S1	CAS	SLARNQP	QHF	Jβ1.5	1
C	B18	265–287		Vβ17S1	CAS	GGADMKTE	AFF	Jβ1.1	1
C	B18	375–397		Vβ2S1	CSA	NPDWGLQLWLH	LRF	Jβ2.1	1
C	B18	375–397		Vβ6S4	CAS	SPGGGKET	QYF	Jβ2.5	1
C	B18	ND		Vβ6S4	CAS	RLRGSSSYNE	QFF	Jβ2.1	1
Total									26

^aThe recognized epitope and restricting HLA molecule are given for each clone. The total number of anti-pp65 or IE1 T-cell clones restricted by a given HLA context for each donor is in boldface. ND, not determined.