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EM analysis of Rep-DNA complexes at protein/DNA mass ratios of 1:1. (A) Rep68H6 protein bound to the center RBE of the 2.7-kb ch-19 integration fragment. (B) Rep68H6 protein bound to the asymmetric RBE of the 1.7-kb DNA fragment. (C) A 953-bp DNA fragment devoid of any RBE sequence and to which Rep68H6 did not bind. Protein binding and EM processing were carried out as described in Materials and Methods. Bar, 0.5 kb.
