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Southern blot analysis of the amplified ch-19 region. HeLa cells were cotransfected with a Rep expression plasmid and Neo construct. Cells were G418 selected, and single colonies were picked and grown up. DNA from individual cell lines was digested with a restriction endonuclease that does not cut within the ch-19 region and was probed with a ch-19-specific probe. Control, untreated HeLa cells; lanes 1 to 3, cell lines derived after transfection with Rep expression plasmids.
