FIG. 9.

Comigration of ch-19 target sequences from primary mouse cells with Rep-Neo vector DNA after analysis by the Southern blot technique. Primary mouse embryo fibroblast (PMEF) cells were infected with a recombinant Rep-Neo AAV virus. Latent cells which were Neo resistant were established as described in Materials and Methods. Lane 1, 10 μg of XbaI-digested ch-19 (PMEF) DNA fractionated on agarose gel, transferred to membrane, and hybridized with a ch-19 sequence-specific probe; lane 2, 10 μg of XbaI-digested ch-19 PMEF DNA was fractionated on agarose, transferred by Southern blotting, and hybridized with a Neo sequence-specific probe.