Table 2. Determination of the Optimum Medium Conditions for Type-1 in YEPD with Varying Temperatures (30–60 °C), pH’s (4.0 and 8.0), and Incubation Periods (24–96 h).
| medium code | incubation period (h) | temperature (°C) | pH | activity (U/mg protein) |
|---|---|---|---|---|
| T1 | 24 | 30 | 4 | 7.45 ± 0.75 |
| T2 | 48 | 30 | 4 | 9.65 ± 1.12 |
| T3 | 72 | 30 | 4 | 6.91 ± 1.07 |
| T4 | 96 | 30 | 4 | 4.47 ± 0.61 |
| T5 | 48 | 30 | 5 | 10.02 ± 0.73 |
| T6 | 48 | 30 | 6 | 12.88 ± 0.98 |
| T7 | 48 | 30 | 7 | 11.61 ± 1.24 |
| T8 | 48 | 30 | 8 | 10.94 ± 0.81 |
| T9 | 48 | 20 | 5 | 9.03 ± 0.64 |
| T10 | 48 | 40 | 5 | 8.47 ± 0.55 |
| T11 | 48 | 50 | 5 | 3.66 ± 0.91 |
| T12 | 48 | 60 | 5 | 1.14 ± 1.52 |