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. 2024 Jun 7;15:1400611. doi: 10.3389/fphys.2024.1400611

TABLE 1.

Yolk fatty acid analyses for eggs from broiler breeder hens fed either a control- or flaxseed-enriched diet during the rearing or laying period. Data are shown as % of total fatty acids. Significant pairwise comparisons (p < 0.05) between eggs laid by hens fed different rearing and laying diets are indicated with differing superscript letters.

Rearing diet
Fatty acid↓/laying diet
Control
Control
Flaxseed
Control
Control
Flaxseed
Flaxseed
Flaxseed
Rearing ANOVA
Laying
Rearing*laying
Alpha-linolenic acid (C18:3 n-3) 1.14 ± 0.12a 1.32 ± 0.13a 2.20 ± 0.15b 3.07 ± 0.05c F = 13.18, p = 0.007 F = 94.40, p<0.001 F = 5.69, p<0.001
Eicosatrienoic acid (C20:3 n-3) 0.08 ± 0.05 0.02 ± 0.00 0.08 ± 0.04 0.06 ± 0.01 F = 1.16, p = 0.31 F = 0.31, p = 0.59 F = 0.31, p = 0.59
Eicosapentaenoic acid (C20:5 n-3) 0.01 ± 0.004 0.03 ± 0.007 0.05 ± 0.003 0.08 ± 0.016 F = 9.33, p = 0.02 F = 32.19, p<0.001 F = 0.19, p = 0.67
Docosapentaenoic acid (C22:5 n-3) 0.18 ± 0.005 0.22 ± 0.019 0.32 ± 0.025 0.45 ± 0.077 F = 2.74, p = 0.14 F = 13.00, p = 0.007 F = 0.77, p = 0.41
Docosahexaenoic acid (C22:6 n-3) 0.87 ± 0.07a 1.10 ± 0.02a 1.46 ± 0.10b 1.89 ± 0.03c F = 44.96, p<0.001 F = 203.01, p<0.001 F = 5.31, p = 0.05
Σ n-3 2.31 ± 0.15a 2.69 ± 0.30a 4.12 ± 0.24b 5.55 ± 0.04c F = 19.97, p = 0.002 F = 131.00, p<0.001 F = 6.62, p = 0.03
Linoleic acid (C18:2 n-6) 25.70 ± 0.74 24.90 ± 0.39 23.66 ± 0.20 24.57 ± 0.70 F = 0.01, p = 0.93 F = 3.08, p = 0.12 F = 1.59, p = 0.24
Gamma-linolenic acid (C18:3 n-6) 0.55 ± 0.15 0.43 ± 0.07 0.40 ± 0.09 0.39 ± 0.36 F = 0.28, p = 0.61 F = 0.59, p = 0.46 F = 0.18, p = 0.24
Eicosadienoic acid (20:2 n-6) 0.55 ± 0.17 0.40 ± 0.06 0.43 ± 0.14 0.36 ± 0.06 F = 0.69, p = 0.43 F = 0.39, p = 0.55 F = 0.09, p = 0.77
Eicosatrienoic acid (20:3 n-6) 0.41 ± 0.19 0.22 ± 0.02 0.36 ± 0.13 0.22 ± 0.04 F = 1.35, p = 0.28 F = 0.03, p = 0.86 F = 0.04, p = 0.84
Arachidonic acid (20:4 n-6) 2.87 ± 0.09 2.68 ± 0.09 2.62 ± 0.14 2.31 ± 0.02 F = 6.48, p = 0.06 F = 0.83, p = 0.03 F = 0.26, p = 0.63
Docosadienoic acid (22:2 n-6) 0 ± 0 0.003 ± 0.003 0.04 ± 0.03 0 ± 0 F = 0.83, p = 0.39 F = 0.83, p = 0.39 F = 1.17, p = 0.31
Σ n-6 30.08 ± 0.81 28.64 ± 0.27 27.51 ± 0.25 27.86 ± 0.57 F = 0.72, p = 0.42 F = 6.77, p = 0.03 F = 1.94, p = 0.20
Σ n-6:Σ n-3 13.16 ± 0.67 10.78 ± 0.79 6.75 ± 0.40 5.02 ± 0.08 F = 9.12, p = 0.02 F = 79.81, p<0.001 F = 0.22, p = 0.65

Bold values represent significant values at p ≤ 0.05.