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. 2024 Jun 21;22:340. doi: 10.1186/s12964-024-01716-5

Fig. 2.

Fig. 2

Triple-negative breast cancer cells adhere closely to neutrophils, facilitating alterations in their function. A Alive co-cultured imaging of neutrophils and tumor cells. Red, Dil-labled neutrophil. Green, GFP. Scale bar, 50 μm. Tumor cells: neutrophils = 1:20. Mice neutrophils were isolated from the bone marrow (BM) of naive mice and human neutrophils were isolated from peripheral blood of patients with benign breast tumor. B Schematic diagram of different co-culture system in vitro. C Flow cytometry analysis of CD11b expression in neutrophils with different interventions for 24 hours. D-E Flow cytometry analysis and quantification of survival dynamics on mice (D) and human (E) neutrophils in different conditioned tumor cell cultured supernatants. Data are presented as the means ± SD from one representative experiment. Similar results were obtained from three independent experiments, unless indicated otherwise. Statistical analysis was performed by one-way ANOVA (C) and Kaplan-Meier analysis (D, E). ns, not significant, *p˂0.05, **p˂0.01, and ***p˂0.001