FIG. 2.
Southern blot analysis of PrV-gE/I/M−Pass. Southern blot analysis was performed with DNAs from wild-type PrV-Ka (lanes 1), PrV-gE/I− (lanes 2), PrV-gM− (lanes 3), PrV-gE/I/M− (lanes 4), and PrV-gE/I/M− passaged 2 (lanes 5), 4 (lanes 6), 6 (lanes 7), 10 (lanes 8), and 20 times (lanes 9) as well the single-plaque isolate PrV-gE/I/M−Pass (lanes 10) after cleavage with BamHI. (A) Ethidium bromide-stained gel. (B) Hybridization with genomic BamHI fragment 7 derived from the US region (see Fig. 4 for location). The locations of the BamHI fragments of PrV wild-type DNA are on the left.