Circulating anti-SIVmac239 Gag antibodies in vaccinated and challenged rhesus monkeys. Animals were immunized at weeks -4, 31, and 58 (open arrows) and challenged (closed arrows) at week 0 with SHIVW6.1D, at week 35 with SHIVhan2 (triangles) or SHIVsf13 (circles), and at week 62 with SHIV89.6p. Groups are as defined in the legend to Fig. 2. For details, see Materials and Methods.