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MHV-H2 mRNA and RF RNA synthesis in CHO and DDT-1 cells. Cultures of CHO and DDT-1 Syrian smooth muscle cell lines were infected with MHV-H2 at an MOI of 10 for 1 h. At 8 and 17 h postinfection, respectively, the cultures were labeled with 300 μCi of 32Pi for 1 h. Intracellular RNA was isolated and treated as described in Materials and Methods and then separated in 0.8% agarose gels for mRNA (A) and RF RNA (B) analysis. Lanes: 1, CHO cells; 2, DDT-1 cells.
