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Construction of pCPV-49. The restriction fragment (nt 1 to 751) of the PV1/M cDNA genome (48) and restriction fragments (nt 752 to 2043; nt 2043 to 7460) of the CVB3/20 genome (52) were ligated in the pES131 vector (48), using the EcoRI (R), HgiAI (H), SacI (S), BglII (B), and ClaI (C) sites to produce a chimeric genome. PV1/M is indicated by the solid bar; CVB3 is indicated by the empty bar. Drawing is not to scale.
