FIG. 2.

One-step growth curve of CPV/49 and CVB3/20 in cell cultures. HeLa (a), HCAEC (b), Panc-1 (c), and MFHF (d) cultures were plated in duplicate and inoculated with either with CPV/49 or CVB3/20 as described in Materials and Methods. Plates were frozen at −74°C at the times shown. Titers were determined on HeLa cell monolayers. □, CPV/49; ■, CVB3/20. (e) Titers of viruses in the three non-HeLa cultures at 24 h p.i. Although virus-inoculated HeLa cell cultures showed typical CPE by 8 to 10 h p.i., no CPE was observed in the other cell cultures used for as long as 24 h p.i.