FIG. 3.

Western blot analysis of CPV/49-infected cell cultures. (A) MFHF cultures were inoculated with CPV/49 (lanes 2 to 5) or CVB3/20 (lanes 6 to 9) at an MOI of 20. MFHF cultures were harvested at 4 (lanes 2 and 6), 7 (lanes 3 and 7), 10 (lanes 4 and 8), or 18 h (lanes 5 and 9) p.i. Lanes 1 and 9, uninfected cells. (B) HeLa cultures were inoculated with CPV/49 (lanes 2 to 4) or CVB3/20 (lanes 5 to 7) at an MOI of 20. HeLa cultures were harvested at 3.5 (lanes 2 and 5), 4.5 (lanes 3 and 6), or 6 (lanes 4 and 7) h p.i. Lane 1, uninfected cells. Ten microliters (equivalent to ca. 4 × 10³ cells) was loaded per lane. A horse polyclonal neutralizing anti-CVB3 antibody was used to detect capsid protein 1D. The HeLa cell blot was exposed to film for 5 s; the MFHF blot was exposed to film for 30 s. Lane 1D, CVB3 capsid protein 1D detected in purified CVB3.