FIG. 2.

Autoradiogram of 12.5% acrylamide-SDS PhastGels showing interaction between DnaB and P2 His-B. Lanes 1 and 2, input of in vitro-synthesized ³⁵S-labeled P2 His-B (lane 1) and control proteins, PinPoint-CAT fusion protein and β-lactamase (called C) (lane 2). Lanes 3 to 6, amounts of P2 His-B and C proteins that do not (lanes 3 and 5) and do (lanes 4 and 6) bind to Q Sepharose beads. Only trace amounts of the proteins are bound to the Q Sepharose. Lanes 7 to 10, Amounts of P2 His-B and C proteins that do not (lanes 7 and 9) and do (lanes 8 and 10) bind to DnaB immobilized on Q Sepharose beads. Almost all of the applied P2 His-B (81%) is present in the bound fraction, while almost all of the applied C proteins (88 and 75%) is found in the nonbound fraction.