TABLE 1.

Bacteria, yeast, and plasmids used

Strain or plasmid	Pertinent features	Reference or source
E. coli
BL21(DE3)	E. coli B strain carrying the T7 RNA polymerase gene under the control of lac promoter	48
DH5Δlac	recA	M. L. Berman
PC2	dnaC2(Ts)	12
S. cerevisiae EGY48	Matα trp1 his3 ura3 lexAops-LEU2	22
Plasmids
pEE853	pEG202 derivative expressing LexA DNA binding domain-DnaB fusion protein	This work
pEE854	pJG4-51 derivative expressing B42 activation domain-P2 B fusion protein	This work
pEE855	pJG4-51 derivative expressing B42 activation domain–P2 B-rlb1 fusion protein	This work
pEG202	lexA DNA binding domain fusion plasmid	22
pET16b	pET plasmid containing the T7 promoter and a His tag	48; Novagen
pJG4-51	B42 activation domain fusion plasmid	22
pRLM109	pBR322 derivative containing the λ P gene under the control of λPL promoter and cI857 repressor	R. McMacken
pSH18.34	lacZ reporter plasmid. Contains eight lexA operators that control lacZ expression	22
pSH17-4	Expresses LexA DNA binding domain-GAL4 activation domain fusion protein	22
pSP102	Mini-P1 plasmid containing P1 ori + initiator gene (P1 coordinates −228 to 1565)	41
pST1	pET16b derivative expressing P2 His-B fusion protein under the control of T7 promoter	This work
pRLM105	pBR322 derivative containing the dnaB gene under the control of λPL promoter and cI857 repressor	R. McMacken