TABLE 3.
Kinetic parameters for RNA synthesis by F3P proteinsa
| Reaction component | Km (nM) | Vmax (fmol) |
|---|---|---|
| Template | ||
| v84 | 0.0125 | 29.9 |
| c84 | 0.0365 | 36.6 |
| Primer (ApG) | 15 | 20.5 |
| Substrate | ||
| ATP | 340 | 18.8 |
| UTP | 18 | 7.5 |
| GTP | 9.3 | 27.4 |
| CTP | 9.3 | 8.9 |
a
The effects of varying the concentrations of templates, primers, and substrates on RNA synthesis in vitro by the 3P complex were analyzed. The amount of 3P complex used was approximately 1.2 pmol each of the three P proteins. The standard reaction mixture included ATP, CTP, and GTP, each at 1 mM; [α-32P]UTP, 0.1 mM; ApG primer, 1 mM; and v84 or c84 template, 10 pmol. Vmax represents the amount of 32P-labeled UTP incorporated under the standard reaction conditions.