FIG. 4.

Partition of the A14.5L-HA protein in the Triton X-114 detergent phase. BS-C-1 cells were infected with vA14.5LHA (10 PFU/cell). After 18 h, the cells were washed with PBS and resuspended in 0.2 ml of 10 mM Tris-HCl, 0.15 M NaCl, and 1% (vol/vol) Triton X-114 (pH 7.4). The phase separation was carried out as described previously (14). Equal volumes of total extract (T), the aqueous phase (A), and the detergent phase (D) were analyzed by SDS-PAGE and Western blotting with MAb 12CA5 or polyclonal antibody to the A17L protein. The positions and masses of marker proteins are indicated to the left.