FIG. 7.

Replication of an A14.5L deletion mutant. B-SC-1 cells in a six-well plate were inoculated with 10 PFU of vaccinia virus strain WR or vA14.5LΔneo/gus per cell for 1 h at 37°C. The cells were then washed twice with medium. At appropriate times, the medium was removed and saved, and the cells were harvested by scraping. The media and cells were centrifuged, and the virus titers in the supernatants of the former (dashed lines) and the pellets of the latter (solid lines) were determined by plaque assay on BS-C-1 cells.