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Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and western immunoblot analyses for IDO in HIV-1-infected and IFN-γ-treated MDM. MDM in culture from donor 1 were either left uninfected (Ctrl) or infected with HIV1-BaL or HIV1-JRFL and cultured for the indicated periods or treated with IFN-γ (600 U/ml) for 48 h. Cultures were homogenized and analysed for IDO as indicated in Materials and Methods.
