KEY RESOURCES TABLE

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rabbit anti-mTOR	Cell Signaling Technology	Cat#: 2983
Rabbit anti-ADMA	Cell Signaling Technology	Cat#: 13522
Rabbit anti-RagA	Cell Signaling Technology	Cat#: 4357
Rabbit anti-pS6K T389	Cell Signaling Technology	Cat#: 9234
Rabbit anti-S6K	Cell Signaling Technology	Cat#: 2708
Rabbit anti-S6	Cell Signaling Technology	Cat#: 2317
Rabbit anti-pS6 S235/236	Cell Signaling Technology	Cat#: 4858
Rabbit anti-p4EBP1 S65	Cell Signaling Technology	Cat#: 9451
Rabbit anti-4EBP1	Cell Signaling Technology	Cat#: 9644
Rabbit anti-NPRL2	Cell Signaling Technology	Cat#: 37344
Rabbit anti-Flag	Cell Signaling Technology	Cat#: 14793
Rabbit anti-HA	Cell Signaling Technology	Cat#: 3724
Rabbit anti-pCAD	Cell Signaling Technology	Cat#: S1859
Rabbit anti-pdS6K	Cell Signaling Technology	Cat#: 9029
Rabbit anti-PRMT1	Proteintech	Cat#: 11279
Rabbit anti-WDR24	Proteintech	Cat#: 20778
Rabbit anti-MTHFD2	Proteintech	Cat#: 12270
Rabbit anti-MAT2A	Proteintech	Cat#: 55309
Mouse anti-β-Actin	Proteintech	Cat#: 20778
Rabbit anti-NPRL3	Abcam	Cat#: ab121346
Rabbit anti-MAT1A	Abcam	Cat#: ab129176
Rabbit anti-ADMA (#2)	Dr. Mark T. Bedford UT MD Anderson Cancer Center	N/A
Rabbit anti-NPRL2 R78me2a	This paper
HRP-conjugated anti-mouse secondary antibody	Sigma-Aldrich	Cat#: A-4416
HRP-conjugated anti-rabbit secondary antibody	Sigma-Aldrich	Cat#: A-4914
Anti-mouse Alexa Fluor 488	Life Technologies/Molecular Probes	Cat#: A11001
Anti-Rabbit Alexa Fluor 594	Life Technologies/Molecular Probes	Cat#: A32740
Bacterial and Virus Strains
XL10 Gold Escherichia coli	Agilent	Cat #200314
BL21(DE3) Escherichia coli	Dr. William G. Kaelin, Jr., Dana-Farber Cancer Institute	N/A
E.coli: One Shot Stbl3 Chemically competent cells	Thermo Fisher Scientific	Cat#C737303
Chemicals, Peptides, and Recombinant Proteins
Anti-FLAG M2 Affinity Gel	Sigma-Aldrich	Cat#: A-2220
Anti-HA M2 Affinity Gel	Sigma-Aldrich	Cat#: A-2095
Full Amino-acids-Deficient Medium	US Biological Life Sciences	Cat#: R8999-04A
Methionine-Deficient Medium	US Biological Life Sciences	Cat#: R8999-06
Leucine-Deficient Medium	US Biological Life Sciences	Cat#: R8998-02
Arginine-Deficient Medium	US Biological Life Sciences	Cat#: R8998-01
Dialyzed FBS	Gibco	Cat#: 26400044
MS023	Selleck Chemicals	Cat#: S8112
Rapamycin	Selleck Chemicals	Cat#: S1039
GSK3368715	Selleck Chemicals	Cat#: S8858
Doxycycline Hyclate	Selleck Chemicals	Cat#: WC2031
L-Methionine hydrochloride solution	Sigma	Cat#: 50272
L-Homocysteine	Sigma	Cat#: 69453
SAH	Sigma	A9384
SAM	Cayman Chemical	13956
Experimental Models: Cell Lines
HEK293	Dr. William G. Kaelin, Jr., Dana-Farber Cancer Institute	N/A
HEK293T	Dr. William G. Kaelin, Jr., Dana-Farber Cancer Institute	N/A
NPRL2Flag HEK293T cells	Dr. David M. Sabatini Whitehead Institute	N/A
NPRL2−/− HEK293T cells	Dr. David M. Sabatini Whitehead Institute	N/A
MDA-MB-231 cells	Dr. Piotr Sicinski Dana-Farber Cancer Institute	N/A
MCF7 cells	Dr. Piotr Sicinski Dana-Farber Cancer Institute	N/A
HCC1500 cells	Dr. Piotr Sicinski Dana-Farber Cancer Institute	N/A
Experimental Models: Organisms/Strains
C57BL/6	Shanghai Laboratory Animal Center	N/A
Recombinant DNA
Tet-on-shPRMT1	This paper	N/A
HA-NPRL2-pRK5	Bar-Peled et al. 2013	Addgene 100513
Flag-NPRL2-pRK5	Bar-Peled et al. 2013	Addgene 46333
HA-NPRL3-pRK5	Bar-Peled et al. 2013	Addgene 46330
HA-DEPDC5-pRK5	Bar-Peled et al. 2013	Addgene 46327
HA-NPRL2-pRK5	Bar-Peled et al. 2013	Addgene 99709
GFP-PRMT9	Hadjikyriacou et al. 2015	Addgene 79675
GFP-PRMT1	Dr. Yanzhong Yang City of Hope	N/A
GFP-PRMT2	Dr. Yanzhong Yang City of Hope	N/A
GFP-PRMT3	Dr. Yanzhong Yang City of Hope	N/A
GFP-PRMT4	Dr. Yanzhong Yang City of Hope	N/A
GFP-PRMT5	Dr. Yanzhong Yang City of Hope	N/A
pLVX-CMV-HA-PRMT1	This paper	N/A
pLVX-CMV-HA-PRMT1-G98R	This paper	N/A
pLVX-CMV-HA-PRMT1-E162Q	This paper	N/A
pLVX-CMV-HA-NPRL2	This paper	N/A
HA-dSamtor	Obio Technology	N/A
Flag-dNprl2	Obio Technology	N/A
Flag-dNprl2-R78F	Obio Technology	N/A
Myc-dDart1	Obio Technology	N/A
TBG-Flag-Nprl2	Obio Technology	N/A
TBG-Flag-Nprl2-R78F	Obio Technology	N/A
TBG-HA-Samtor	Obio Technology	N/A
TBG-Flag-Nprl2	Obio Technology	N/A
TBG-Flag-Prmt1	Obio Technology	N/A
TBG-Flag-Prmt1-G98R	Obio Technology	N/A
TBG-Flag-Prmt1-E162Q	Obio Technology	N/A
TBG-shMat1a	Obio Technology	N/A
TBG-shSamtor	Obio Technology	N/A
TBG-shNprl2	Obio Technology	N/A
TBG-shPrmt1-3’UTR	Obio Technology	N/A
Oligonucleotides
PRMT1-CDS shRNA targeting sequence	GTGTTCCAGTATCTCTGATTA	N/A
PRMT1-3’UTR shRNA targeting sequence	TGAGCGTTCCTAGGCGGTTTC	N/A
PRMT1 sgRNA targeting sequence #1	AAAGCCAACAAGTTAGACCA	N/A
PRMT1 sgRNA targeting sequence #2	GATGGCCGTCACATACAGCG	N/A
PRMT1 sgRNA targeting sequence #13	GAGGCTCATCCCATTAGCCA	N/A
SAMTOR sgRNA targeting sequence	GAAATACTGCTCGTGCGCAG	N/A
NPRL2 sgRNA targeting sequence	GATGCGGCAGCCGCTGCCCA	N/A
MAT2A sgRNA targeting sequence	TTAAAGGAGGTCTGTGCCGG	N/A
Mat1a (mouse) shRNA targeting sequence	GCAGGATAATGGTGCAGTCAT	N/A
Samtor (mouse) shRNA targeting sequence	GATGTTGGCAGCTGCTTTAAT	N/A
sidDart1 sequence	GCAGCGAGGAUACAUACAATT	N/A
sidNprl2 sequence	TGGGTTTGTTGTAGTTTGTAA	N/A
sidSamtor sequence	TGGAATCCTACAGAGCCGAGGG	N/A
F-alpha-dtubulin	5’-CAACCAGATGGTCAAGTGCG-3’	N/A
R-alpha-dtubulin	5’-ACGTCCTTGGGCACAACATC-3’	N/A
F-dSamtor	5’-GACCAACGATGGGAAGGTGG-3’	N/A
R-dSamtor	5’-GCTCTGTAGGATTCCAGGAGT-3’	N/A
F-dDart1	5’-TGAGGGAGTGGACATTATTATTTCC-3’	N/A
R-dDart1	5’-TATGTGTTCGACTCCTGAATTTGAG-3’	N/A
F-dNprl2	5’-GGCTGCAAGATAAGCTGCCA-3’	N/A
R-dNprl2	5’-GCGTTGAAGATGCTGCTTGG-3’	N/A
F-mouse Srebp1c	5’-GGAGCCATGGATTGCACATT-3’	N/A
R-mouse Srebp1c	5’-GGCCCGGGAAGTCACTGT-3’	N/A
F-mouse Scd1	5’-GCAAGCTCTACACCTGCCTCTT-3’	N/A
R-mouse Scd1	5’-CGTGCCTTGTAAGTTCTGTGGC-3’	N/A
F-mouse Pepck	5’-CGATGACATCGCCTGGATGA-3’	N/A
R-mouse Pepck	5’-TCTTGCCCTTGTGTTCTGCA-3’	N/A
F-mouse G6p	5’-GAAGGCCAAGAGATGGTGTGA-3’	N/A
R-mouse G6p	5’-TGCAGCTCTTGCGGTACATG-3’	N/A