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A Western blots and B–H quantitative analysis of GSDMD-FL, GSDMD-N, NLRP3, IL-1β, IL-18, CD31, and VEGF-α protein levels in ischemic gastrocnemius muscles of WT mice compared to non-ischemic controls at the 2nd, 5th, 7th, and 14th day post-HLI. n = 3 for each group; ns represents p > 0.05, *p < 0.05, **p < 0.01, ***p < 0.001, #p < 0.0001.
