FIG. 2.

Drug susceptibility of cDNA-derived MRL-1237- and guanidine-resistant mutants. The effect of 2C mutations on drug susceptibility was examined with MRL-1237 and guanidine hydrochloride by plaque-reduction assay. A wild-type [PV1(M)] and drug-resistant [PV1(M)2C-F164Y, PV1(M)2C-I120V, PV1(M)2C-N179A, or PV1(M)2C-N179G] mutants derived from cDNA were inoculated to HeLa cells. After adsorption, HeLa cell monolayers were incubated for 72 h without drug (solid bars), with 2 mM guanidine (striped bars), or with 50 μM of MRL-1237 (open bars).