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Effects of pH on the steady-state kinetic parameters of the PaDADH E246L variant enzyme with D-leucine as substrate at 25 oCa
| Kinetic parameter | CH | pKa | R2 |
|---|---|---|---|
| kcat/Km | 2200 ± 500 | 8.7 ± 0.1 | 0.987 |
Enzymatic activities were measured at varying concentrations of D-leucine and fixed 1 mM PMS. Reactions were carried out in 20 mM sodium pyrophosphate. The kcat/Km parameter values were obtained after fitting the kinetic data with Equation 4.
