Bone marrow culture medium
| Reagent | Final concentration | Amount |
|---|---|---|
| DMEM (high glucose) | N/A | 45 mL |
| Heat-Inactivated FBS | 10% | 5 mL |
| Penicillin streptomycin | 1% | 500 μL |
| M-CSF | 20 ng/mL | 1000 ng |
| Total | N/A | 50 mL |
Storage conditions: 4°C for 1 week.
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