T cell culture Medium

Reagent	Final concentration	Amount
RPMI-1640	N/A	45 mL
FBS	10%	5 mL
Penicillin streptomycin	1%	500 μL
HEPES (1 M)	N/A	500 μL
2-mercaptoethanol (1000×)	N/A	50 μL
Non-essential amino acids MEM (100×)	N/A	500 μL
IL-2	100 units/mL	5000 units
Total	N/A	50 mL

Storage conditions: 4°C for 1 week.