Protein lysis Buffer
| Reagent | Final concentration | Amount |
|---|---|---|
| 10×RIPA | N/A | 10 μL |
| 10×PhosSTOP | 10% | 10 μL |
| 25×Protease inhibitor cocktail | 1% | 4 μL |
| ddH2O | 20 mmol/L | 76 μL |
| Total | N/A | 100 μL |
Storage conditions: 4°C for 1 day.
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