PCR cycling conditions
| Step | Temperature | Duration | Cycles |
|---|---|---|---|
| UDG activation | 50°C | 2 min | Hold |
| Dual-Lock DNA Polymerase | 95°C | 2 min | Hold |
| Denature | 95°C | 15 s | 40 |
| Anneal/extend | 60°C | 1 min |
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