Incomplete Lysis Buffer (25 mM HEPES, pH 7.5, 150 mM NaCl, 0.2% (v/v) Triton X-100, 0.02% (v/v) TWEEN-20)
| Reagent | Final concentration | Amount |
|---|---|---|
| 500 mM HEPES | 25 mM | 25 mL |
| 5.0 M NaCl | 150 mM | 15 mL |
| 20% (v/v) aqueous Triton X-100 solution | 0.2% (v/v) | 5 mL |
| 20% (v/v) aqueous TWEEN-20 solution | 0.02% (v/v) | 0.5 mL |
| MilliQ water | N/A | 500 mL |
Store at 4°C for up to 3 months.