Reagent	Source
Tris Base	Fisher Bioreagents (Pittsburgh PA, USA)
Sodium Chloride	Fisher Chemical (Pittsburgh PA, USA)
Glycerol	Pharmco (Brookfield CT, USA)
Biotin	IBA Lifesciences (Göttingen Germany)
Tris(2-carboxyethyl)phospine hydrochloride (TCEP-HCl)	GoldBio (St. Louis MO, USA)
Luria-Bertani (LB) Media	Fisher Scientific (Waltham MA, USA)
Ampicillin	GoldBio (St. Louis MO, USA)
Isopropyl β- d-1-thiogalactopyranoside (IPTG)	Fisher Scientific (Waltham MA, USA)
Iron (III) Chloride Hexahydrate	Strem Chemicals Inc. (Newburyport MA, USA)
Calcium Chloride	Acros Organics (Geel, Belgium)
Manganese (II) Chloride Tetrahydrate	Fisher Chemical (Pittsburgh PA, USA)
Zinc Sulfate Heptahydrate	Fisher Scientific (Waltham MA, USA)
Cobalt (II) Chloride Hexahydrate	TCI (Tokyo, Japan)
Nickel (II) Chloride Hexahydrate	Chem-Impex Intl Inc. (Wood Dale IL, USA)
Sodium Molybdate Dihydrate	Fisher Chemical (Pittsburgh PA, USA)
Sodium Selenate Pentahydrate	Honeywell Research Chemicals (Morris Plains NJ, USA)
Boric Acid	Sigma-Aldrich (Darmstadt, Germany)
Aprotinin	GoldBio (St. Louis MO, USA)
Leupeptin	Alfa Aesae (Ward Hill MA, USA)
Pepstatin A	Fisher Scientific (Waltham MA, USA)
AEBSF	GoldBio (St. Louis MO, USA)
Phenylmethylsulfonyl fluoride	GoldBio (St. Louis MO, USA)
PEG3350	Sigma-Aldrich (Darmstadt, Germany)
Sucrose	Avantor Materials (Radnor PA, USA)
Dithiothreitol (DTT)	GoldBio (St. Louis MO, USA)
Sodium Dodecyl Sulfate (SDS)	Fisher Bioreagents (Pittsburgh PA, USA)
Bromophenol Blue	GoldBio (St. Louis MO, USA)
40% Acrylamide:Bisacrylamide Solution (19:1)	Fisher Bioreagents (Pittsburgh PA, USA)
N, N, N’, N’-tetramethylethylenediamine (TEMED)	Fisher Bioreagents (Pittsburgh PA, USA)
Ammonium Persulfate (APS)	Sigma-Aldrich (Darmstadt, Germany)
Urea	Fisher Chemical (Pittsburgh PA, USA)
Ethylenediaminetetraacetic acid (EDTA)	Fisher Bioreagents (Pittsburgh PA, USA)
Imidazole	Sigma-Aldrich (Darmstadt, Germany)
Sybr Gold	Invitrogen (Carlsbad CA, USA)
NEB Buffer 2.1	New England Biolabs (Ipswich MA, USA)
Cell Strain	Genotype	Source
E. coli BL21 HMS174 (DE3)	F - recA1 hsdR (r K12 - m K12 + ) (Rif R )	Novagen
Plasmids	Genotype	Description
2R-T PaCasDinG	pET-StrepII-dinG	pET N-terminal Strep II tag Pseudomonas aeruginosa CasDinG expression plasmid.
Oligonucleotides	Sequence	Source
Top Strand	5’-TCGTCACCAGTACAAAC-3’	Integrated DNA Technologies (IDT)
Bottom Strand	5’-TTTTTTTTTTTTTTTTGTTTGTACTGGTGACGA-3’	Integrated DNA Technologies (IDT)