FIG. 3.

Induction of MxA RNA by IFN-α is impaired in HPV31 cells. (A) Northern blot analysis of 8 μg of total RNA from NHK or LKP31 cells treated with IFN-α (100 or 500 U/ml) with MxA cDNA as a probe. MxA RNA signals in panel A were quantified by a PhosphorImager, and the results are shown graphically in panel B. The induction of MxA RNA by IFN-α reaches comparable levels in LKP31 cells and NHKs at a higher dose (1,000 U/ml) and longer time exposure. (C) Northern blot analysis of 10 μg of total RNA from NHKs and LKP31 cells treated with IFN-α with MxA cDNA as a probe. MxA RNA signals in panel C were quantified by a PhosphorImager, and the results are shown graphically in panel D.