Fig. 7.

Phylogeny of Gcm in metazoans. Among non-bilaterians, Gcm is conserved only in the Cnidaria. Support values > 50 are shown at basal nodes. Nematostella Gcm is framed in red. Species screened for Gcm are listed in Supplementary Table 2. Bidirectional BLAST [199, 200] searches using the Drosophila melanogaster Gcm1 protein sequence were performed against databases of metazoan organisms. Fungal protein sequences with the highest similarity to animal Gcm were used as an outgroup. Sequences were aligned using MUSCLE (Mega7) and trimmed by eye, to include only the domain. The tree was constructed with the maximum likelihood (ML) method using PhyML (SeaView). Bootstrap support is based on 2000 replicates