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. 2024 Jun 21;19:10. doi: 10.1186/s13064-024-00184-4

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 8 — Phylogeny of Gcm in Anthozoa (Cnidaria). This domain is highly conserved in stony corals and sea anemones. Support values > 50 are shown at basal nodes. Nematostella Gcm is framed in red. Cnidarian species screened for Gcm are listed in Supplementary Table 3. Bidirectional BLAST [199, 200] searches using Drosophila melanogaster Gcm1 protein sequence were performed using databases of metazoan organisms. Fungal protein sequences with the highest similarity to animal Gcm were used as an outgroup. Sequences were aligned using MUSCLE (Mega7) and trimmed by eye to include only the domain. The tree was constructed with the maximum likelihood (ML) method using PhyML (SeaView). Bootstrap support is based on 2000 replicates