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. 2024 Jun 22;23:192. doi: 10.1186/s12944-024-02175-0

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Effects of 7-MEGA™ on lipid accumulation of 3T3-L1 adipocytes and adipogenesis-related markers. A Chemical structure of palmitoleic acid. B Cell viability of 3T3-L1 adipocytes treated with increasing concentrations (125, 250 and 500nL/mL) of 7-MEGA™, as determined by the WST-1 assay. C Representative images of Oil Red O staining showing adipocyte differentiation after treatment with 125, 250 and 500nL/mL of 7-MEGA separately. D Quantification of lipid accumulation in adipocytes treated with different concentrations of 7-MEGA™ using the Oil Red O staining assay. E, F Western blot analysis of protein expression levels of adipogenesis-related markers, PPARγ, C/EBPα, SREBP-1, FABP4, FAS, ACC, and SCD-1 in 3T3-L1 adipocytes treated with increasing concentrations of 7-MEGA™. Densitometric quantification of protein expression levels normalized to loading control (β-actin). Data are presented as mean ± SD. *** P < 0.001 VS. the control group