Table 3.

Formulations, Critical Aggregation Concentrations (CACs) and Nanofiber

Formulation	Peptide Ratio (Molar Ratio)	CAC (μM)	Zeta Potential (mV)	Nanofibers Dimensions (Length; Diameter)
P1+P2+P3	50:45:5	12.8± 0.1	+14.1±0.6	150±50 nm; ~10 nm
P1+P2+P3+P6	50:44:5:1	17.5±0.3	+8.9±0.3	500±100 nm; ~10 nm
P1+P2+P3+P6	50:42.5:5:2.5	17.3±0.2	+15.5±0.4	230±50 nm; ~10 nm
P1+P2+P3+P6	50:40:5:5	11.3± 0.1	+1.7±0.3	125±45 nm; ~15–20 nm

Abbreviations: AFM, Atomic Force Microscopy; BC, Breast Cancer; C19, Nonadecanoic acid; CAC, Critical Aggregation Concentration; CD, Circular Dichroism; Dox, Doxorubicin; EGFR, Epidermal Growth Factor Receptor; FTIR, Fourier-Transform Infrared Spectroscopy; HER2, Human Epidermal Growth Factor Receptor 2; HFIP, 1,1,1,3,3,3-Hexafluoro-2-propanol; MMP-9, Matrix Metalloproteinase 9, NR, Nile Red; PAs, Peptide Amphiphiles; SEM, Scanning Electron Microscopy; TEM, Transmission Electron Microscopy; ThT, Thioflavin T; TNBC, Triple-negative breast cancer;