Figure 4.

AMPK(Thr172) phosphorylation, SIRT1, PGC1α, citrate synthase protein content in myotubes from lean and severely obese subjects in response to 24 h of resveratrol treatment (Resv). A: AMPK (Thr172) phosphorylation. B–D: SIRT1, PGC1α, and citrate synthase protein content. Following detecting significance for either group, treatment, and/or interaction effect using three-way ANOVA, distinguishing meaningful differences were performed using a two-tailed Student’s t test. There were main effects (P < 0.05) for resveratrol for pAMPK(Thr172) for both groups. There was a significant interaction effect (P < 0.05) for subject group with resveratrol for SIRT1. Data are presented as means ± SE. n = 8 per group (all females); *P < 0.05 for selected comparison. CTR, control.