TABLE 3.
Analysis of the cloned cDNA products from the control in vitro RT-PCR reactions using single or mixed BMV RNA3 templatesa
| RNA template(s) | Total no. of clones analyzed | Restriction profileb
|
|||||
|---|---|---|---|---|---|---|---|
| Parental | SF23 | SF25 | SF29 | SF30 | Recombinants | ||
| Single | |||||||
| SF23 | 3 | 3 | |||||
| SF25 | 14 | 14 | |||||
| SF29 | 3 | 3 | |||||
| SF30 | 10 | 10 | |||||
| Mixed | |||||||
| SF23 × SF25 | 11 | 4 | 7 | 0 | |||
| SF23 × SF29 | 14 | 12 | 2 | 0 | |||
| SF23 × SF30 | 11 | 4 | 7 | 0 | |||
| SF29 × SF30 | 8 | 3 | 5 | 0 | |||
a
The table summarizes the results of the control in vitro recombination experiments during the RT-PCR amplifications using the indicated transcribed BMV RNA3 templates (see Materials and Methods for experimental details).
b
The numbers represent the number of the individual types of recombinants (indicated) that have been identified as having the particular type of restriction profile.