Table 3. ANDV infectivity assay.
Percentage of infection detected by anti-N protein antibody using flow cytometry after inoculation of Vero E6 cells with ANDV-RNA positive samples collected during the acute phase of infection.
| Incubation time | Sample | Number | % Infected cells range | Number of samples >1% infection | Number of samples >2% infection |
|---|---|---|---|---|---|
|
| |||||
| 8 hpi | GCF | 25 | 1–9 | 10 | 3 |
| NPS | 7 | 1–3 | 4 | 1 | |
| Saliva | 10 | 1–13 | 3 | 1 | |
| Urine | 1 | 2 | 1 | 1 | |
| Total | 43 | 18 | 6 | ||
|
| |||||
| 48hpi | GCF | 10 | 0–15 | 8 | 6 |
| NPS | 4 | 1–3 | 4 | 4 | |
| Saliva | 3 | 4–5 | 3 | 3 | |
| Urine | 1 | 9 | 1 | 1 | |
| Total | 18 | 16 | 14 | ||