Fig. 4. VZV gC enhances IFN-γ-induced phosphorylation of STAT1 and its nuclear translocation.
a HaCaT cells were stimulated with 5 ng/mL IFN-γ, 300 nM VZV gCS147-V531 or both and phosphorylation levels of STAT1 (pSTAT1) at Y701 were detected. A representative immunoblot out of three independent ones with its respective loading control (TCE) is depicted, as well as a graph showing the pSTAT1/TCE signal ratios for the 30 min time points (n = 3 biological replicates). The three uncropped blots from the biological replicates are shown in Source Data. The graph shows the mean ± SD and the asterisk indicates statistical significance following a two-sided unpaired t-test with Welch’s correction. b HaCaT cells were treated with either 5 ng/mL of IFN-γ produced in bacteria or mammalian cells (mIFN-γ) in the presence or absence of 300 nM gCS147-V531. pSTAT1 was detected by immunofluorescence. Representative graphs show nuclear pSTAT1 signal for the two different time points tested (10 or 30 min) from one out of two independent biological replicates (n = 2 biological replicates), except for mIFN-γ, which was performed only once. Each dot represents a nucleus and at least 140 nuclei per condition were analysed through Cell Profiler. Error bars indicate mean ± SD and asterisk indicates statistical significance following one-way ANOVA with Bonferroni’s post hoc test (*P < 0.05; ***P < 0.001). Data were provided as Source Data.